产品货号：
Z22182
中文名称：
NEU2抗体
英文名称：
Anti-NEU2 Antibody
产品规格：
100μg
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-NEU2 Antibody |
| 指标别名 | Sialidase-2;3.2.1.18;Cytosolic sialidase;N-acetyl-alpha-neuraminidase 2;NEU2 |
| 克隆性 | Polyclonal |
| 检验物种 | human |
| 应用范围 | ELISA,WB |
| 基因名称 | NEU2 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion near aa 100-125 of Human Neu2. |
| 计算分子量 | 42kDa |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.This antibody reacts with human Neu2.Based on sequence we expect this antibody to react with neuraminidase from other sources,although specific reactivity has not been confirmed.Cross-reactivity against Neu1 has not yet been established.Neuraminidases are highly conserved in mammals and therefore cross-reactivity is expected with mouse and rat Neu2. |
| 浓度 | 0.9mg/mL by UV absorbance at 280nm |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | Neuraminidases or sialidases are exoglycosidases that catalyze the cleavage of汐-glycosidically linked terminal N-acetyl neuraminic acid from sialylated glycoconjugates.They are widely spread in nature,occurring in viruses,bacteria,fungi,protozoa,birds and mammals.Together,the neuraminidases form a family of hydrolases that share a conserved active site and similar sequence motifs.Three types of neuraminidase are found in mammals and are defined as lysosomal,plasma membrane and cytosolic on the basis of their biochemical properties and subcellular distribution.Lysosomal N-acetyl-汐-neuraminidase (NEU1) has significant primary structure characteristics of other mammalian and microbial sialidases with similar substrate specificity.However,unlike other members of this family,lysosomal neuraminidase requires the carboxypeptidase protective protein/cathepsin A (PPCA) for intracellular transport and lysosomal activation.The enzyme is only catalytically active when it is bound to PPCA and is a component of a high molecular weight,multi-protein complex containing PPCA,?-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase.Using a hamster Sial3 probe,Monti et al.(1999) identified the gene encoding sialidase-2,which they designated NEU2,from a human genomic library.The 2 putative exons of NEU2 encode a deduced 380-amino acid protein with a calculated molecular mass of 42.23kD.The NEU2 protein has significant homology with the mammalian,viral,and bacterial sialidases.It shares over 72% similarity with the hamster and rat cytosolic sialidases and over 42% similarity with human NEU1.NEU2 contains a potential N-linked glycosylation site,2 aspartic acid block consensus sequences,and an N-terminal F/YRIP sequence motif which is part of the active site of other sialidase enzymes.Monti et al.hypothesized that NEU2 has a cytosolic localization because it does not contain a cleavage site,transmembrane domain,or targeting motifs. |
| Uniprot ID | Q9Y3R4 |
| 基因名全称 | Sialidase-2 |
| 推荐稀释比 | ELISA:1:10,000 - 1:50,000 IHC:User Optimized IP:User Optimized WB:1:500- 1:2,000 |
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